pcr-script® cam cloning kit (Agilent technologies)
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Agilent technologies
pcr-script® cam cloning kit
Pcr Script® Cam Cloning Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr-script+cam+cloning+kit/us10351629-445-14-18
Average 90 stars, based on 1 article reviews
Pcr Script® Cam Cloning Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr-script+cam+cloning+kit/us10351629-445-14-18
Average 90 stars, based on 1 article reviews
pcr-script® cam cloning kit - by Bioz Stars,
2026-09
90/100 stars
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Amplification:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Purification:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Polymerase Chain Reaction:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Clone Assay:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Produced:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Electrophoresis:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Gel Extraction:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Agarose Gel Electrophoresis:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Extraction:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Transformation Assay:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Software:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Recombinant:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the Plasmid Preparation:Article Title: AmpC ?-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Monoclonal antibodies specific for human interferon-alpha subtype alpha 8 Article Snippet: The DNA fragments amplified with the Ex Taq kit were blunted according to “Polishing the Purified PCR Products” as a manual for handling of Article Title: CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity Article Snippet: The nucleotide and the deduced protein sequences were analyzed by using software available at the National Center for Biotechnology Information website ( http://www.ncbi.nlm.nih.gov ). β-Lactamase gene cloning. bla CTX-M-93 was amplified by using primers CTX-M-ATG and CTX-M-stop ( ) and cloned directly into the Article Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula. Article Snippet: PCR products were cloned into the pPCR-Script Cam SK(+) vector according to the manufacturer’s instructions for the Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK(+) cloning vector using a Article Title: Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet (O)-Mediated Tetracycline Resistance Article Snippet: The conditions were 94°C for 3 min, followed by 30 cycles of 94°C for 30 s, 42°C for 45 s, and 68°C for 2 min. PCR products (1,628 kb) were purified, and the ends of purified PCR products were polished to generate blunt ends and cloned into pPCR-Script Cam SK( ) cloning vector using a Article Title: AmpC β-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins Article Snippet: The amplification product was cloned using a Article Title: Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes Article Snippet: The 1,710-bp PCR product was polished with the |